Antioxidative effect of hormonal replacement therapy in women with lipids metabolism disturbances

Lipids peroxidation, especially LDL cholesterol is well known effect of reactive oxygen intermediates (ROI) activity in organism. Lipids peroxidation products have ahterogenic activity, increasing ischaemic heart disease risk
Aim of study: Comparison of antioxidative effect of hormonal replacement therapy in groups of women with lipids metabolism disturbances and normal lipids values.
Material and methods: Study group consists of 29 women with hipercholesterolaemia (mean age 54.3±5.0) and 30 women with hiperlipidaemia (hipercholesterolaemia or hipertriglyceridaemia (mean age 54.6±4.8). Control group includes 27 women with normal lipids concentrations (mean age 53.8±5.2).
ROI generation was evaluated in 20 ml peripheral blood samples. Spontaneous ROI generation and generation after neutrophils acivation with stimulators: formyl-metionyl-leucylphenyloalanin (fMLP), phorbol acetate (PMA) and zymosan was measured. As an enhancer of chemiluminescency luminol was used. Chemiluminescency associated with oxydation burst was measured with Luminometr 1251, BioOrbit, Turku, Finland in stable temperature during 30 minutes (15 readings). Measurements were performed before applying continuous estrogen-progestin hormonal replacement therapy, after 3 and 6 months of this therapy.
Results: Evaluating HRT influence on ROI generation in mentioned groups in case of some chemiluminescence parameters, interactions between HRT duration and ROI generation were observed. During HRT decrease of ROI generation was faster in groups with hipercholesterolaemia and hipertriglyceridaemia than in women with normal lipids concentrations. This interactions do not allow to conclude general relations.
Conclusion: Hormonal therapy antioxidative effect does not depend on lipids metabolism disturbances.